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Enzyme technology was a very promising field in modern biotechnology. Its application was widely recognised by various industries like food and beverages, detergent and laundry, healthcare, textile, leather, to name a few. Various businesses were taking advantage of the newer genetic engineering techniques to develop and produce enzymes with enhanced properties. Global enzymes market was expected to grow at CAGR of 6.3% to reach a whopping figure of USD7 billion in 2017. It was further estimated to grow on an average rate of 6.5% through 2022. Even though enzyme technology had proved to be beneficial in providing environment friendly and sustainable processes to the companies, it possessed several challenges. Enzyme market was a technology intensive industry, manufacturers were required to invest consistently in innovation and technology to improve efficiency of their products. Specialty enzyme market also faced constraints due to cheaper alternatives and lack of awareness among consumers. Further, biofuel enzymes market had witnessed a stagnant demand; however, biofuel production from cellulosic biomass showed potential for growth. The case study analyses the advances of enzyme technology in various industries and ponders if enzyme technology would make a mark in the industrial applications around the globe.